abstract：:Experimental evidence accumulated to date by several research groups indicates that antisense oligodeoxynucleotides targeted against messenger RNA (mRNA) sequences located downstream of the initiation codon fail to inhibit the translation of this mRNA unless the hybrid is cleaved by RNase H. It has previously been sho...

journal_title：Biochimie

authors： Cazenave C,Frank P,Büsen W

更新日期：1993-01-01 00:00:00

abstract：:Recombinant plasmids containing head-to-tail copies of different coat-protein replacement genomes of wheat dwarf virus (WDV) were used to study the mechanism leading to the release of replicating unit-length molecules in suspension culture cells of Triticum monococcum. For plasmids bearing two complete genomes, the vi...

journal_title：Biochimie

authors： Heyraud F,Matzeit V,Schaefer S,Schell J,Gronenborn B

更新日期：1993-01-01 00:00:00

abstract：:This paper deals with the discovery of plasmalogen deficiency in the cerebro-hepato-renal (Zellweger) syndrome and discusses how this has led to the development of postnatal and prenatal diagnostic procedures for this and a number of related peroxisomal disorders in man that show a general impairment in the biosynthes...

journal_title：Biochimie

authors： van den Bosch H,Schrakamp G,Hardeman D,Zomer AW,Wanders RJ,Schutgens RB

更新日期：1993-01-01 00:00:00

abstract：:Adrenoleukodystrophy (ALD) is an X-linked peroxisomal disorder characterized by a progressive demyelination of the central nervous system and adrenal insufficiency. Clinical phenotypes of different severity are frequently observed within the same kindred. ALD is characterized biochemically by the accumulation of very-...

journal_title：Biochimie

authors： Aubourg P,Mosser J,Douar AM,Sarde CO,Lopez J,Mandel JL

更新日期：1993-01-01 00:00:00

abstract：:Using a rapid FPLC procedure for the separation of calpain I, calpain II and the inhibitor calpastatin from pork meat, results of proteolytic and inhibitory activity were obtained. Duplicate measurements showed variation coefficients less than 0.13. Compared to others we found lower calpain I activity 1 h post-mortem....

journal_title：Biochimie

authors： Iversen P,Ertbjerg P,Larsen LM,Monllao S,Møller AJ

更新日期：1993-01-01 00:00:00

abstract：:The nucleotide sequence of the gene encoding chicken ribosomal protein L7a was determined. The gene contains eight exons and seven introns, which spread over 3613 nucleotides. The transcription initiation sites were located on four consecutive nucleotides GCCC, which are at the 5' terminus of a short polypyrimidine tr...

journal_title：Biochimie

authors： Maeda N,Kenmochi N,Tanaka T

更新日期：1993-01-01 00:00:00

abstract：:The central glycolytic and oxidative pathways and the ATP-producing mechanisms differ in sane and malignant cells by their regulation and dynamics. Fast-growing, poorly-differentiated cancer cells characteristically show high aerobic glycolysis. In the same way, cholesterol biosynthesis, which occurs by normal pathway...

journal_title：Biochimie

authors： Baggetto LG

更新日期：1992-11-01 00:00:00

abstract：:Two-dimensional J-resolved and correlated 1H NMR spectra with complete water suppression have been obtained to further characterize a metabolic pattern for normal and pathological human plasma samples. 1H COSY spectra have been recorded on plasma from 12 patients with cancer in order to check for the possible presence...

journal_title：Biochimie

authors： Kriat M,Confort-Gouny S,Vion-Dury J,Viout P,Cozzone PJ

更新日期：1992-09-01 00:00:00

abstract：:Recently a method was proposed which permits the extraction of the exact interatomic distance information from the measurement of the evolution of a single cross-peak relative to the mixing time in a NOESY experiment. This is performed through a careful multi-exponential analysis allowing the extraction of the relaxat...

journal_title：Biochimie

authors： Reisdorf C,Malliavin TE,Delsuc MA

更新日期：1992-09-01 00:00:00

abstract：:The hbs operon of Bacillus subtilis comprises a single gene which is localized between the spoIVA and mtrA open reading frames, and is situated at 204 degrees of the standard map of B subtilis. Expression of hbs is initiated from two distinct promoters called P1 and P2. The transcription initiation sites have been map...

journal_title：Biochimie

authors： Micka B,Marahiel MA

更新日期：1992-07-01 00:00:00

abstract：:The product of the spoIIM gene of Bacillus subtilis is required for complete septum migration and forespore engulfment during sporulation. To investigate whether expression of spoIIM is required in the forespore compartment of the sporangium, we have constructed a new integrational vector, pKSV7, which contains temper...

journal_title：Biochimie

authors： Smith K,Youngman P

更新日期：1992-07-01 00:00:00

abstract：:Inhibition of polypeptide chain elongation with the mRNA-complementary (antisense) oligonucleotide has been realized through a RNase H independent mechanism. Nuclease resistant complementary non-natural alpha-17-mer oligonucleotide did not inhibit cell-free protein biosynthesis of beta-globin in the wheat germ system ...

journal_title：Biochimie

authors： Boutorine AS,Boiziau C,Le Doan T,Toulmé JJ,Hélène C

更新日期：1992-05-01 00:00:00

abstract：:The wheat germ translation initiation factor 2 (WGeIF-2) was isolated in a homogeneous state by an efficient procedure and characterized. Its molecular mass, as determined by a gel-filtration method is approximately 150,000 Da. According to SDS-PAGE WGeIF-2 consists of four subunits with M(r) 37,000 (alpha), 40,000 (b...

journal_title：Biochimie

authors： Shaikhin SM,Smailov SK,Lee AV,Kozhanov EV,Iskakov BK

更新日期：1992-05-01 00:00:00

abstract：:In parallel with crystallographic studies of ribosomes from Thermus thermophilus, a long-term program on the crystallization and structural investigations of ribosomal proteins from the same microorganism has been started at the Institute of Protein Research (Pushchino, Russia). At present, more than half of the indiv...

journal_title：Biochimie

authors： Garber MB,Agalarov SC,Eliseikina IA,Fomenkova NP,Nikonov SV,Sedelnikova SE,Shikaeva OS,Vasiliev D,Zhdanov AS,Liljas A

更新日期：1992-04-01 00:00:00

abstract：:The effect of curing agents (salt, nitrate, ascorbic acid and glucose) and processing parameters (pH, water activity and drying and cooking temperatures) on pork muscle cathepsins B, D, H and L as well as leucyl, arginyl and tyrosyl hydrolysing activities is reported. Salt (60 g/l) showed a powerful inhibitory effect,...

journal_title：Biochimie

authors： Toldrá F,Rico E,Flores J

更新日期：1992-03-01 00:00:00

abstract：:Administration of beta-adrenergic agonists to domestic species can lead to skeletal muscle hypertrophy, probably by reducing the rate of myofibrillar protein breakdown. Myofibrillar breakdown is associated with the calcium-dependent proteinase system (calpains I,II and calpastatin) whose activity also changes during b...

journal_title：Biochimie

authors： Bardsley RG,Allcock SM,Dawson JM,Dumelow NW,Higgins JA,Lasslett YV,Lockley AK,Parr T,Buttery PJ

更新日期：1992-03-01 00:00:00

abstract：:So far, no efficient affinity chromatography for CCK receptor purification has been reported that prevented obtention of sequenceable amounts of purified receptor. In this work, 10% of plasma membrane receptor sites were specifically cross-linked with the photoreactive cleavable agonist 125I-ASD-[Thr28, Ahx31]-CCK-25-...

journal_title：Biochimie

authors： Dufresne M,Poirot S,Jimenez J,Vaysse N,Fourmy D

更新日期：1992-02-01 00:00:00

abstract：:A neoglycolipid of structure beta-D-Glcp-S-(CH2)3N(OH)(CH2)4-O-cholest-5-en-3 beta-yl has been prepared in fair overall yield by reduction of the nitrone obtained by condensation of beta-D-Glcp-S-(CH2)3NHOH and OCH-(CH2)3-O-cholest-5-en-3 beta-yl. This synthetic procedure is very flexible, allowing a large range of le...

journal_title：Biochimie

authors： Tronchet JM,Zsély M,Ricca A,Barbalat-Rey F,Geoffroy M

更新日期：1992-01-01 00:00:00

abstract：:The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subu...

journal_title：Biochimie

authors： Cornut B,Willson RC

更新日期：1991-12-01 00:00:00

abstract：:We have previously identified a protein with Mr approximately 40,000 (p40) that binds with high specificity and affinity to the 5'-untranslated leaders of mitochondrial mRNAs in yeast. Here we show that this protein is abundant, comprising about 0.4% of total mitochondrial protein. p40 is present in a cytoplasmic (rho...

journal_title：Biochimie

authors： Dekker PJ,Papadopoulou B,Grivell LA

更新日期：1991-12-01 00:00:00

abstract：:We report Raman spectra of various cholinesterases: lytic tetrameric forms (G4) obtained by tryptic digestion of asymmetric acetylcholinesterase (AChE) from Torpedo californica and Electrophorus electricus, a PI-PLC-treated dimeric form (G2) of AChE from T marmorata, and the soluble tetrameric form (G4) of butyrylchol...

journal_title：Biochimie

authors： Aslanian D,Grof P,Bon S,Masson P,Négrerie M,Chatel JM,Balkanski M,Taylor P,Massoulié J

更新日期：1991-11-01 00:00:00

abstract：:The molecular basis for the essential role of cholesterol in mammalian (and other cholesterol-requiring) cells has long been the object of intense interest. Cholesterol has been found to modulate the function of membrane proteins critical to cellular function. Current literature supports two mechanisms for this modula...

journal_title：Biochimie

authors： Yeagle PL

更新日期：1991-10-01 00:00:00

abstract：:The major pterin in Tetrahymena pyriformis, strain W, earlier suggested to be L-threo-biopterin and named ciliapterin [1] is now identified as D-threo-neopterin (D-monapterin). This is the first example of a natural D-monapterin. This compound was characterized by its chromatographic behavior, its fluorescence propert...

journal_title：Biochimie

authors： Klein R,Tatischeff I,Tham G,Grolière CA

更新日期：1991-10-01 00:00:00

abstract：:Elongation factor 1 alpha (EF-1 alpha) was purified to homogeneity from full-grown oocytes of Xenopus laevis. This protein is encoded by a gene previously shown to be expressed in male and female germ cells, and repressed in somatic cells. The purified protein was identified with EF-1 alpha on criteria of molecular ma...

journal_title：Biochimie

authors： Morales J,Mulner-Lorillon O,Denis H,Bellé R

更新日期：1991-09-01 00:00:00

abstract：:Oligonucleotides that complement Escherichia coli 16S ribosomal RNA residues 685-696 and 694-705 have been synthesized so as to incorporate antibody-recognizable markers: a 3'-terminal residue of N6-delta 2-isopentenyladenosine, a 5'-dinitrophenyl group, or both. Each oligonucleotide is able to bind RNA within the sma...

journal_title：Biochimie

authors： McWilliams RA,Glitz DG

更新日期：1991-07-01 00:00:00

abstract：:We have modified elongation factor Tu (EF-Tu) from Escherichia coli via mutagenesis of its encoding tufA gene to study its function-structure relationships. The isolation of the N-terminal half molecule of EF-Tu (G domain) has facilitated the analysis of the basic EF-Tu activities, since the G domain binds the substra...

journal_title：Biochimie

authors： Anborgh PH,Cool RH,Gümüsel F,Harmark K,Jacquet E,Weijland A,Mistou MY,Parmeggiani A

更新日期：1991-07-01 00:00:00

abstract：:The complete amino acid sequences of 3 proteins from the 50S subunit of Bacillus stearothermophilus ribosomes were determined by N-terminal sequence analysis and by sequencing of overlapping fragments obtained from enzymatic digestions and chemical cleavages. The proteins BstL28, BstL33 and BstL34, named according to ...

journal_title：Biochimie

authors： Kruft V,Kapp U,Wittmann-Liebold B

更新日期：1991-07-01 00:00:00

abstract：:Three trends are seen in the organization of ribosomal DNA genes during evolution: 1) gradual separation and separability of the regulation of transcription of 5S and larger subunit rRNAs; 2) retention of a transcription unit containing both large and small rRNAs; and 3) clustering of genes for both 5S and 18S-28S rDN...

journal_title：Biochimie

authors： Srivastava AK,Schlessinger D

更新日期：1991-06-01 00:00:00

abstract：:The targets of in vivo studies of the ribosomal assembly process are mainly the events of rRNA processing, whereas in vitro studies (total reconstitution) focus on principles of the assembly process such as assembly-initiation proteins, rate-limiting steps and a detailed sequence of assembly reactions (assembly map). ...

journal_title：Biochimie

authors： Nierhaus KH

更新日期：1991-06-01 00:00:00

abstract：:The gene for the ribosomal protein L5 from the archaebacterium Sulfolobus acidocaldarius has been isolated and sequenced. The gene codes for a basic protein of molecular weight 29 165 Da. This protein shows substantial similarity to the equivalent protein from other archaebacteria as well as from yeast, and considerab...

journal_title：Biochimie

authors： Yang D,Günther I,Matheson AT,Auer J,Spicker G,Böck A

更新日期：1991-06-01 00:00:00

abstract：:The maximum activities of some key enzymes of metabolism were studied in lungs of fed and 48-h-starved rats. The maximum activity of hexokinase in the lung is similar to that of other tissues of the body, but lower than that of phosphorylase and 6-phosphofructokinase. High activities of glucose-6-phosphate dehydrogena...

journal_title：Biochimie

authors： Ardawi MS

更新日期：1991-05-01 00:00:00

abstract：:The physical maps of cloned recBCD gene regions of Serratia marcescens and Proteus mirabilis were correlated to genes located in this region. The genes thyA, recC, recB, recD and argA were organized as in Escherichia coli. The 3 rec genes code for the 3 different subunits of the RecBCD enzyme and produced enzymes prom...

journal_title：Biochimie

authors： Rinken R,de Vries J,Weichenhan D,Wackernagel W

更新日期：1991-04-01 00:00:00

abstract：:The SOS response is induced in E coli following treatments that interfere with DNA replication. The response is under the control of the recA and the lexA genes. Strains defective in LexA repressor constitutively express SOS proteins. However, SOS repair does not reach its maximum level in these strains. Instead, an a...

journal_title：Biochimie

authors： Lesca C,Petit C,Defais M

更新日期：1991-04-01 00:00:00

abstract：:The chemical carcinogen N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) induces frameshift mutations located within two types of specific sequences (mutation hot spots): i) contiguous guanine sequences and ii) alternating GC sequences. The genetic requirements of these frameshift events were investigated using specific ...

journal_title：Biochimie

authors： Janel-Bintz R,Maenhaut-Michel G,Takahashi M,Fuchs RP

更新日期：1991-04-01 00:00:00

abstract：:The RecA protein of E coli promotes a strand exchange reaction in vitro which appears to be similar to homologous genetic recombination in vivo. A model for the mechanism of strand transfer reaction by RecA protein has been proposed by Howard-Flanders et al based on the assumption that the RecA monomer has two distinc...

journal_title：Biochimie

authors： Horii T

更新日期：1991-02-01 00:00:00

abstract：:The recF gene of E coli lies within a cluster of genes which play essential roles in DNA replication; the gene order is dnaA dnaN recF gyrB. Each of these genes has its own promoters which, with the exception of dnaA promoters, reside entirely within the translated region of the respective preceding gene. In this repo...

journal_title：Biochimie

authors： Pérez-Roger I,García-Sogo M,Navarro-Aviñó JP,López-Acedo C,Macián F,Armengod ME

更新日期：1991-02-01 00:00:00

abstract：:A major incentive in inhibitor research is that control of limited proteolysis constitutes a valuable pharmacological tool. Protease inhibitors have proved to be successful in influencing pathogenesis in many experimental models but a breakthrough to use in human therapy has mainly been restricted to aprotinin and ang...

journal_title：Biochimie

authors： Scharpe S,De Meester I,Hendriks D,Vanhoof G,van Sande M,Vriend G

更新日期：1991-01-01 00:00:00

abstract：:During the suckling period, the rats are fed continuously with milk, which is a high-fat low-carbohydrate diet (HF). At weaning, milk is progressively replaced by the rat's laboratory chow which is a high-carbohydrate low-fat diet (HCHO), and this is accompanied by large hormonal modifications: an increase in plasma i...

journal_title：Biochimie

authors： Girard J,Perdereau D,Narkewicz M,Coupé C,Ferré P,Decaux JF,Bossard P

更新日期：1991-01-01 00:00:00

abstract：:Seven virulent bacteriophages of Streptococcus thermophilus were characterized at the molecular level and classified into 2 subgroups (A and B) by DNA/DNA hybridization experiments and analysis of their structural proteins. Two representatives of subgroups A and B were compared to 3 representatives of Neve's subgroups...

journal_title：Biochimie

authors： Benbadis L,Faelen M,Slos P,Fazel A,Mercenier A

更新日期：1990-12-01 00:00:00

abstract：:A synthetic gene of yeast aspartic acid tRNA with a promoter for phage T7 RNA polymerase was cloned in Escherichia coli. The in vitro transcribed tRNA(Asp) molecules are deprived of modified nucleotides and retain their aspartylation capacity. The solution conformation of these molecules was mapped with chemical struc...

journal_title：Biochimie

authors： Perret V,Garcia A,Puglisi J,Grosjean H,Ebel JP,Florentz C,Giegé R

更新日期：1990-10-01 00:00:00